“…..there is little information on the effect of chronic caffeine consumption on exercise adaptations. We first sought to characterize the effect of caffeine on protein synthesis in vitro using immortalized C2C12 and TTD6 muscle and tendon cells.”
“Total protein synthesis, as measured by puromycin incorporation, decreased 31% in TTD6 cells with 4 mM caffeine and 41% in C2C12 cells with 0.5 mM caffeine.”
“The structure and function of in vitro engineered ligaments were also reduced with caffeine, including a 45% reduction in maximal tensile load (P = 0.0128) and 30% reduction in collagen content.”
“The structure and function of in vitro engineered ligaments were also reduced with caffeine, including a 45% reduction in maximal tensile load and 30% reduction in collagen content…”
“Mice that consumed caffeine while exercising did not gain skeletal muscle mass (gastrocnemius and soleus) to the same extent as the noncaffeinated exercising mice.”
“Together, these data suggest that high caffeine consumption can dampen the molecular signals associated with protein synthesis and in excess may limit exercise-induced skeletal muscle adaptations.”
“In mice, muscle mass gains with 6 wk of exercise are limited with a high physiological dose of caffeine (roughly equivalent to a human dose of 5.7 mg/kg).”
D. Stiffen, et al
Caffeine decreases muscle and tendon protein synthesis and engineered ligament strength in vitro and attenuates adaptation to exercise in mice
Journal Of Applied Physiology — Volume 139 #6 — December 2025 — page 1569